https://nova.newcastle.edu.au/vital/access/ /manager/Index ${session.getAttribute("locale")} 5 https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:37665 2 ≥ 0.997). However, TruCount beads produced the most consistent (concentration variation = 3.8%) calculated numbers of plasma CD41⁺/Annexin V⁺ MV, which were significantly higher from that calculated using either Flow-Count or CountBright (p < 0.001). The FACSCanto was able to resolve 0.5 μm beads by FSC and 0.16 μm beads by SSC, but there were significantly more background events using SSC compared with FSC (3113 vs. 470; p = 0.008). In general, sample analysis by SSC resulted in significantly higher numbers of MV (p < 0.0001) but was well correlated with enumeration by FSC for all MV subtypes (ρ = 0.62–0.89, p < 0.0001). We conclude that all counting beads provided linear results at concentrations ranging from 6 beads/μl to 100 beads/μl, but TruCount was the most consistent. Using SSC to gate MV events produced high background which negatively affected counting bead enumeration and overall MV calculations. Strategies to reduce SSC background should be employed in order to reliably use this technique.]]> Tue 09 Mar 2021 18:12:55 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:34397 in vitro. Notably, only the first eluted MP fraction bound HK₂ cells indicating a possible association between MP size and cell‑targeting properties.]]> Tue 03 Sep 2019 18:26:10 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:24678 in vivo and to determine the underlying mechanism by which simvastatin reduces hepatic steatosis.]]> Sat 24 Mar 2018 07:10:52 AEDT ]]>